O-291 Clinical Application of Noninvasive PGT for Patients with Recurrent Pregnancy Loss or Repeated Implantation Failure

Abstract Study question Has noninvasive PGT been allied with the patients experiencing recurrent pregnancy loss (RIF) and implantation failure (RPL)? What are clinical beneficiaries? Summary answer Embryo selection through can reduce miscarriage rate in RPL improve RIF. is known already Chromosomal abnormalities exist early human embryos, especially Therefore, embryos usually evaluated pre-implantation genetic testing for aneuploidy (PGT-A). However, a biopsy an important concern undetermined health risks. Meanwhile, researchers observed genomic DNA contents embryo culture medium. Since then, multiple studies have published using medium analyzing chromosomal ploidy. In 2016, Xu et al. first reported chromosome screening (NICS) assay spent blastocyst Nevertheless, application of NICS has not or design, size, duration We designed retrospective cohort study including 303 subjects from July 2018 to May 2021, according records Reproductive Centre at Second Affiliated Hospital Wenzhou Medical University. Patients RIF who received were included group, while those underwent conventional morphology transfer during same period non-NICS group. Participants/materials, setting, methods women history (≥2 pregnancies) (≥3 implantations), exclusion criteria antiphospholipid syndrome (APS), diabetes, hypothyroidism, other severe complications. Routine IVF/ICSI was performed based on sperm quality. The placed droplets. Approximately 30 mL each transferred into cell lysis buffer. Whole-genome amplification media, followed by library preparation ChromInst (Yikon Genomics; EK100100724 Inst Library Preparation Kit). Main results role chance For RPL, per FET significantly lower group than (17.9% vs. 42.6%), whereas ongoing (40.7% 25.0%) live birth (38.9% 20.6%) higher compared no differences identified rates patient between groups (49.6% 44.9%). RIF, non- (46.9% 28.7%), significant difference (23.3% 25.9%). Limitations, reasons caution As study, had different prognoses groups, introducing bias results. Wider implications findings Non-invasive be used only detection but also comprehensive evaluation concentration, mosaic ratio, resolution, etc. accumulation outcomes combined data as index predict embryos. Trial registration number NA